Investigating the role of ARM protein in plant growth and development
Téma již má řešitele.- Řešitel
- Jiří Kopřiva - Gymnázium Brno, Křenová, příspěvková organizace
- Instituce
- Biofyzikální ústav AV ČR, v. v. i.
- Další údaje o pracovišti
- Department of Cell Biology & Radiobiology
- Lektoři
- AGOSSA ANICET BATCHO
- Podpora
- JCMM podpořila toto SOČ téma částkou 20 000 Kč na materiál a částkou 10 000 Kč na honorář školitele.
Title
- Investigating the role of ARM protein in plant growth and development
Plant growth and development are complex processes modulated by numerous genes, transcription factors, hormones and peptides. Telomerase is an essential eukaryotic enzyme that maintains chromosomal DNA ends and has implications in cancer and aging. The human telomerase complex is well characterized. However, plant telomerase is much less well understood. Recently, the protein ARM, was identified as a telomerase interaction partner in Arabidopsis thaliana. This project is a fundamental research on the regulation of plant growth. To obtain significant results contributing to the understanding of the interaction mechanisms of the ARM protein in plant growth and development, we intend to apply a molecular and genetic approach. By combining these approaches, we want to show whether the ARM protein is involved in plant responses to osmotic and salt stress. Students will learn the best methods that we routinely use in our laboratory using Arabidopsis thaliana as model plant. They will also be familiar and experienced in methods of plant molecular biology & physiology through different experiments:
1. Seed germination experiment
Aim of the experiments is to determine seed germination sensitivity to salt stress of arm gene in comparison with cv. Col
2. Growth experiment
Aim of the experiment is to determine responsiveness of hypocotyl and root sensitivity to salt stress
3. Gene Expression experiment:
Analyse the expression of arm gene in seedling under salinity stress. Aim of the experiment is to investigate if light influence seeds/seedling tolerance to salt stress and whether salinity can influence light signalling in arm seedlings.